“At the start, restriction enzymes were very important in isolating DNA fragments. These days, they’re more often used to diagnose whether you made the recombinant DNA properly or not,” Roberts said. ...

Restriction enzymes are one of the most important tools in the recombinant DNA technology toolbox. But how were these enzymes discovered? And what makes them so useful? Aa Aa Aa When I come to the ...

Gateway is based on lambda phage recombinase; the enzyme, which Invitrogen calls Clonase™, swaps DNA fragments flanked by att sites, making it possible to move fragments between Gateway- compatible ...

AFLP-PCR uses many of the same steps as RFLP, SSR, and RAPD, including restriction enzyme digestion of genomic DNA, PCR, and electrophoretic separation of DNA fragments. However, this method ...

Prepare the restriction enzyme digests. You will perform two single digests using ... pre-weigh a sterile Eppendorf tube in which you will collect the gel slice containing the desired DNA fragment.

A restriction map of the 5.3 kb insert is prepared. The recombinant plasmid is digested with the enzymes listed above in single digests, and then several combinations of enzymes are tested in double ...

This process is referred to as cloning. It uses restriction enzymes to cut the plasmid and the gene fragment at sequence-specific locations that depend on the enzyme used. Then, enzymes called ligases ...