The absorbance at 412 nm was detected to measure the GSH/GSSG ratio with standard curve. Cells were collected and incubated with 5 μM C11 BODIPY (581/591) (invitrogen, D3861) for 30 minutes at 37 ℃.
Laboratory for Chemistry and Life Science, Institute of Integrated Research, Institute of Science Tokyo, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan ...
Key Laboratory of Colloid and Interface Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong 250100, China ...