The absorbance at 412 nm was detected to measure the GSH/GSSG ratio with standard curve. Cells were collected and incubated with 5 μM C11 BODIPY (581/591) (invitrogen, D3861) for 30 minutes at 37 ℃.
Laboratory for Chemistry and Life Science, Institute of Integrated Research, Institute of Science Tokyo, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan ...
Key Laboratory of Colloid and Interface Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong 250100, China ...
Adipogenic differentiation of the GIPCs was evaluated by Oil Red O and BODIPY staining, and the expression changes of adipogenic genes like ACC, ATGL, CEBPβ, PPARγ, etc. Related mechanisms were ...
EVs were purified by differential centrifugation as described (20). For fluorescent observations, EVs were labeled using 50 μM of thiol reactive BODIPY FL maleimide or BODIPY TR dyes (Thermo Fisher ...
The rate of prion replication in cells is controlled by a gene regulatory network that is associated with glycosaminoglycan sulphation and activation of metalloproteinases. Retinoic acid ...
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