Design and results mRNA expression of 17 nutrient ... RNA quantity and quality was assessed using a NanoDrop. RT-PCR was performed as previously described.16 Primers (see online supplementary table S1 ...

PCR-Machines are based on Polymerase Chain Reaction (PCR) and produce copies of genes exponentially. The PCR cycling reactions are carried out in an automated thermocycler, which is capable of ...

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Equal amounts of total RNA were then reverse-transcribed, and cDNA was subjected to real-time quantitative (q)PCR (Westburg, Leusden, The Netherlands) or to micro-array analysis. Details on real-time ...

The Primer Design Tool is a powerful bioinformatics application designed to help researchers and scientists create highly optimized primers for PCR (Polymerase Chain Reaction) experiments. Built using ...

College of Food and Biological Engineering, Hefei University of Technology, Hefei, Anhui 230601, China ...