Primer3-py also includes bindings for the Primer3 primer design engine if you'd prefer to use an established pipeline. The IO parameters mirror those of the original Primer3. **Please note that while ...

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The primers for bisulfite PCR or nested PCR were designed by MethPrimer (Li and Dahiya, 2002) or PyroMark Assay Design 2.0, and were listed as follows: cg02261780 (GNA11): Outer Pair: OF: 5′TTA GGT ...