The absorbance at 412 nm was detected to measure the GSH/GSSG ratio with standard curve. Cells were collected and incubated with 5 μM C11 BODIPY (581/591) (invitrogen, D3861) for 30 minutes at 37 ℃.
Laboratory for Chemistry and Life Science, Institute of Integrated Research, Institute of Science Tokyo, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan ...
Key Laboratory of Colloid and Interface Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong 250100, China ...
The work by Vallier and colleagues programmes ESCs and IPSCs towards hepatocyte fate by using a combination of hepatocyte transcription factors. Based on informatic analyses comparing adult ...
Adipogenic differentiation of the GIPCs was evaluated by Oil Red O and BODIPY staining, and the expression changes of adipogenic genes like ACC, ATGL, CEBPβ, PPARγ, etc. Related mechanisms were ...
EVs were purified by differential centrifugation as described (20). For fluorescent observations, EVs were labeled using 50 μM of thiol reactive BODIPY FL maleimide or BODIPY TR dyes (Thermo Fisher ...
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