gondii real-time PCR assay for the diagnosis of ocular toxoplasmosis. This single-reaction, dual-target design maximises inclusivity and sensitivity, without loss of specificity. The authors would ...

The procedure requires specialized thermal cyclers with fluorimeters. Multiplex PCR 3 Multiplex PCR uses multiple primers to amplify different targets in a single reaction. This technique saves time ...

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We carried out multiplex small RNA sequencing (MSR-seq ... periodate oxidation to block unligated hairpin oligonucleotide; 9) second ligation of PCR primer; 10) PCR using Illumina index primers. For ...

The PCR-Tm analysis system was optimized and modified based on four parts: the concentration of each untagged primer, each tagged primer ... The PCR-Tm analysis is a single closed-tube multiplex PCR ...

With this Multiplex CRISPR (CRISPRm) system, we selected an improved cellobiose utilization pathway in diploid yeast in a single round of mutagenesis and selection, which increased cellobiose ...