Science News, Educational Articles, Expert Opinion | The Scientist
Bio-Rad Extends Range of Vericheck ddPCR Empty-Full Capsid Kits to Optimize AAV Vector Characterization. Bio-Rad Laboratories, Inc. (NYSE: BIO and BIO.B), a global leader in life science research and clinical diagnostics products, today announced the launch of Vericheck ddPCR™ Empty-Full Capsid Kits for adeno-associated virus (AAV) serotypes 2 and 8, enabling determination of capsid titer ...
What Does a Positive Covid Test Look Like? | The Scientist
May 11, 2024 · ABOVE: A positive rapid test will yield two visible lines (middle) while a valid negative test will only show a single line in the control region (right) and an invalid test will show no control line (left). iStock, ASIFE V iral TikTok videos showing teenagers hacking rapid COVID-19 tests by inserting sodas and other acidic solutions into the tests’ inlet caused quite a …
Universe 25 Experiment | TS Digest | The Scientist
May 28, 2024 · UNIVERSE 25: John Calhoun crouches within his rodent utopia-turned-dystopia that, at its peak, housed approximately 2,200 mice. Calhoun was studying the breakdown of social bonds that occurs under extreme overcrowding, following up on his earlier discovery of the learned "behavioral sink" phenomenon in rats. 1
The Cre- loxP System: A Powerful Tool in the Genetic Toolbox
Sternberg pondered about the underlying mechanism; he suspected that there must be a genetic hotspot for recombination located at the terminal ends of the linear genome to facilitate plasmid cyclization. 1. The genesis of Cre-loxP recombinationConsistent with his hypothesis, Sternberg’s experiments revealed a small fragment of P1 DNA at the end of …
Insights into qPCR: Protocol, Detection Methods, and Analysis
Nov 8, 2023 · Like PCR, qPCR amplifies DNA using a DNA polymerase enzyme.This adds new nucleotides to the 3’-OH end of a short, single-stranded DNA segment, called a primer, which complements the template strand. 2 The reaction occurs in specialized thermal cyclers equipped with fluorometers that measure fluorescent signals, quantifying both relative and absolute DNA amounts when combined with reference ...
Infographic: The Cre-lox System Explained | The Scientist
Dec 4, 2023 · C re-loxP recombination allows scientists to excise, insert, or invert specific DNA segments with unprecedented accuracy. 1,2 This works through two key components: a Cre recombinase and loxP sequence recognition sites. The Cre enzyme identifies pairs of loxP sites (arrowheads), which flank (flox) the DNA, and catalyzes reciprocal DNA recombination between the two sites to excise a small piece ...
Introduction to Bioprocessing | The Scientist
May 8, 2024 · Upstream and Downstream Bioprocessing. Bioprocesses encompass a sequence of activities that facilitate biochemical changes, with upstream processing steps that prepare biomass and substrates for catalytic reactions, followed by downstream processing to handle the resulting materials. 1 To change the phase or composition of raw materials, most systems employ a series of methods called unit ...
Biochemistry Articles | The Scientist Magazine®
The Scientist's articles under the category: Biochemistry. Researchers examine how long-term exposure to widely used per- and polyfluoroalkyl substances (PFAS) detrimentally affect human health.
Optimizing PCR: Proven Tips and Troubleshooting Tricks
Feb 23, 2024 · Tanuka Biswas is a science communication professional, with over a decade of experience in molecular biology, cancer biology, and content strategy. She earned her PhD in biomedical sciences at the University of Texas Health Science Center, with a focus on cancer biology. She is passionate about ...
Microbiology Articles | The Scientist Magazine®
The Scientist's articles under the category: Microbiology. Scientists uncover the mechanism by which gut bacteria convert molecules in bile into sex hormones.
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